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. 2004 Feb 24;90(6):1244–1251. doi: 10.1038/sj.bjc.6601656

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Copyright © 2004 Cancer Research UK

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RT–PCR was performed with primers spanning exons 11–15 of the BRCA2 gene (nucleotides 6948–7714; GenBank accession number NM_000059) on RNA extracted from lymphocytes as described by Claes et al (2003). RT–PCR on RNA from the patient carrying BRCA2 R2336H (lane 1) showed a full-length fragment of 767 bp and three faster migrating bands (697 bp: skipping of out of frame exon 13 (stop 2345); 671 bp: skipping of in-frame exon 12 (deletion of 32 amino acids); 601 bp: skipping of out of frame exons 12 and 13 (stop 2311)). In healthy control persons (lanes 2 and 3), besides the full-length fragment, a band (671 bp) representing the skipping of in-frame exon 12, was also observed. M, marker.