Figure 4.

Lung metastases in mice immunised with TNF autovaccine in the B16F10 melanoma model. C57BL/6 mice (4-week old) (n=12/treatment group) were immunised i.m. with 100 μg TNF autovaccine or PBS in CFA and boosted three times at two-weekly intervals in IFA. B16F10 cells (10⁵) in 200 μl of PBS were injected into their tail veins at 4 weeks post initial immunisation. At 7 weeks, the mice were killed and the lungs excised. The numbers of lung metastases were counted (A) and six randomly selected tumours measured with calipers (B). Each group was compared to the PBS control and the one-Way ANOVA test was used to evaluate results. The mean and P-values for each group are shown. A representative of three independent experiments is shown, ^*P<0.01.