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. 2004 Feb 24;90(6):1252–1258. doi: 10.1038/sj.bjc.6601680

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Copyright © 2004 Cancer Research UK

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Transduction efficiency of DOSPA/DOPE or TAT-pK with chloroquine in various human cancer cell lines. Cells (5 × 10⁴) were incubated with 1 ml medium containing pGL3-promoter DNA (1 μg) complexed with DOSPA/DOPA (open bars) or with TAT-pK in the presence of 50 μM chloroquine (grey bars) or 100 μM chloroquine (filled bars) and grown as described under Materials and Methods. After 48 h incubation, cells were harvested and luciferase activity was measured. Each end point represents the mean±s.d. RLU, relative light units.