Table 2. Summary of the GPC3 promoter methylation data of tumour cells and their GPC3 mRNA expression status.
|
Methylation status |
||||||||
|---|---|---|---|---|---|---|---|---|
|
PCR-based assaya |
Southern blot-based assayb |
|||||||
| Sample | Origin | Genderc | Expressiond | Proximal sites | Distal sites | EagI | SacII | BssHII |
| SK-N-AS | NB cell line | F | +++ | − | tr | − | − | − |
| SK-N-SH | NB cell line | F | ++ | − | tr | tr/− | tr/− | +/− |
| SK-N-DZ | NB cell line | F | − | + | + | +/− | +/− | +/− |
| SJNB-1 | NB cell line | M | ++ | − | − | − | − | − |
| SJNB-7 | NB cell line | M | +++ | − | − | − | − | − |
| SJNB-10 | NB cell line | M | +++ | − | − | ND | ND | ND |
| NBL-S | NB cell line | M | ++ | − | − | ND | ND | ND |
| IMR-32 | NB cell line | M | ++ | − | − | ND | ND | ND |
| SK-N-FI | NB cell line | M | − | − | − | − | − | − |
| NB4 | Primary NB | F | + | + | + | tr/− | tr/− | tr/− |
| NB5 | Primary NB | F | + | − | tr | − | tr/− | tr/− |
| NB8 | Primary NB | F | ND | + | + | ND | ND | ND |
| NB11 | Primary NB | M | − | − | − | − | − | − |
| NB13 | Primary NB | M | + | − | − | − | − | − |
| NB193 | Primary NB | M | ND | − | − | ND | ND | ND |
| WT130 | Primary WT | F | ++ | + | + | +/− | +/− | +/− |
| WT42 | Primary WT | F | + | + | + | +/− | +/− | +/− |
| WT51 | Primary WT | F | ++ | − | + | − | − | − |
| WT106 | Primary WT | F | + | + | ND | ND | ND | ND |
| WT40 | Primary WT | M | +++ | − | − | − | − | − |
| WT177 | Primary WT | M | ++ | − | tr | +/− | +/− | +/− |
| WT116 | Primary WT | M | + | tr | − | ND | ND | ND |
| WT158 | Primary WT | M | ND | tr | + | ND | ND | ND |
GPC3=glypican 3; PCR=polymerase chain reaction; NB=neuroblastoma; WT=Wilms' tumour.
a
+=methylated; −=not methylated.
b
+=methylation signal; −=nonmethylation signal; tr=traces; +/−=both methylation and nonmethylation signals; tr/−=both traces of methylation signal and nonmethylation signal; ND=not determined.
c
F=female; M=male.
d
Transcriptional expression: −=no expression; +=weak expression; ++=moderate expression; +++=strong expression. The analysis of the expression levels was reported in Saikali et al (2000) and was based on the visual inspection of all the Northern blots or semiquantitative RT–PCR assays.