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. 2004 Mar 23;90(8):1636–1643. doi: 10.1038/sj.bjc.6601706

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Copyright © 2004 Cancer Research UK

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Monocytes incubated with GM-CSF and IL-4 developed immature DCs on day 7. After infection with (A) recombinant adenovirus (Ad661) or (B) without infection, and incubation with MCM and poly [I] : poly [C], the mature DCs were developed within 48 h. The expression of DC maturation markers was determined by FACS with the cells gated in the scatter profile. (C) The DCs (have also been transfected by Ad661) decreased their endocytosis ability after maturation. Endocytosis was determined by analysis of FITC-dextran internalisation at 37 and 4°C (negative control).