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. 2004 Mar 23;90(8):1636–1643. doi: 10.1038/sj.bjc.6601706

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Copyright © 2004 Cancer Research UK

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Intracellular flow cytometry assay for detection of IFN-γ-expressing CD8⁺ T lymphocytes in the PBMCs of HLA-A2⁺ donor. The proportion of IFN-γ-secreting CD8⁺ T cells are indicated as percentage in the bidimenional flow cytometry diagram. (A) Effector cells with HepG2 cells; (B) effector cells only.