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. 2004 Mar 23;90(8):1620–1626. doi: 10.1038/sj.bjc.6601723

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Copyright © 2004 Cancer Research UK

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Effect of ephrin-B2 overexpression on tumour vessel counts and blood volume in tumours. (A) Immunohistochemical staining for CD31 in tumour sections was performed. The mean vessel count in tumours from mice injected with ephrin-B2-sense-transfected KM12L4 cells (white bars) was significantly greater than in control tumours (black bars) (^*P<0.0001, ANOVA). Error bars indicate s.e.m. of the mean. HPF, high-power field. (B) ⁵¹Cr-labelled RBCs suspended in HBSS were injected into the tail vein of tumour-bearing nude mice. The mean blood volume in tumours from ephrin-B2-sense-transfected KM12L4 cells (S2) was significantly less than in control tumours from vector-transfected cells (^*P=0.0288, nonparametric test). Error bars indicate s.e.m. of the mean.