Table 1. Primers used in HDSS.
|
Tag counta |
||||||
|---|---|---|---|---|---|---|
| Sequence (GTAC–) | FC | FA | Gene bank match | Selected 3′primer (CGCGCGT13) | Resultb | |
| F1 | CTGCCGCCGCCGCC | 0 | 61 | Cellular retinoic acid-binding protein | -GC | B |
| F2 | TCGGGGAGCTGCGG | 1 | 27 | No match | -GC | B |
| F3 | TATTTAACTGACTA | 0 | 18 | Tumour rejection antigen (gp96) 1 (TRA1) | -CT | C |
| F4 | GTGGGCCTGTCTGC | 5 | 74 | Trefoil factor 3 | -CG | A |
| F5 | CAGCCGGGCCAGCA | 1 | 13 | Cell membrane glycoprotein, 110000M(r) | -GC | B |
| F6 | TCCAGCGCTGGGAG | 0 | 9 | No match | -GA | B |
| F7 | CGGGAGAGGCAGGT | 0 | 9 | Oxidative 3 alpha hydroxysteroid dehydrogenase | -GC | B |
| F8 | CTTCCCACTGGCCT | 0 | 8 | Histone deacetylase 1 (HDAC1) | -CA | B |
| F9 | CGGCCCCCGCCGCC | 0 | 7 | cDNA, 3′ end (Hs.205805) | -GC | B |
| F10 | CCAACGGGCCGGGG | 0 | 7 | No match | -GC | B |
| F11 | ACTGCAGCCTCCAA | 0 | 7 | Interferon, alpha-inducible protein | -GT | B |
| F12 | CTGGCATGGCAGCA | 0 | 6 | GSTT1 | -GT | C |
| F13 | TGGGTCTGTTGGCC | 0 | 5 | SUMO-1 activating enzyme subunit 1 (SAE1) | -GC | B |
| F14 | TGGGCACAGGCCGC | 0 | 5 | (Clone hKvBeta3) K+ channel beta subunit | -GA | B |
| F15 | GACGAGTCGGGCCC | 0 | 5 | Actin, gamma 1 (ACTG1) | -GG | D |
| F16 | CTTGCGGCCCGTGT | 0 | 5 | No match | -GA | B |
| F17 | CTGCGGCCGCCTCG | 0 | 5 | cDNA DKFZp566J153 | -GC | D |
| F18 | CTCAGAAGTGTGCC | 0 | 5 | No match | -GC | D |
| F19 | CGATGCGTGGGCTG | 0 | 5 | TM7XN1 protein | -GA | C |
| F20 | CCTGCATCACTGCC | 0 | 5 | No match | -GC | C |
| C1 | TTCGCCGAGAGGGT | 15 | 1 | Cysteine-rich protein 1 (intestinal) (CRIP1) | -CC | B |
| C2 | CGCCGCCGCGCCGC | 12 | 0 | No match | -GG | C |
| C3 | CTGACTTGGGCATC | 22 | 2 | SNC73 protein (SNC73) | -GG | B |
| C4 | CTCACGCTTATCAG | 10 | 1 | No match | -CA | C |
| C5 | CCCAGACGTCTGGT | 10 | 0 | No match | -CC | B |
| C6 | TTTCAACCATCTAC | 6 | 0 | No match | -CC | B |
| C7 | CTTTGAACAGCAAC | 6 | 0 | Unknown mRNA | -CA | C |
| C8 | CTGCTGCTCAAGCC | 6 | 0 | Clone 333H23 on chromosome 22q12.1–12.3. | -GT | B |
| C9 | CGGAAGCGAGAGTG | 6 | 0 | EST(BM782041) | -GG | B |
| C10 | CCGGGAGGCGGAGG | 6 | 0 | Chromosome 16 BAC clone | -GA | B |
| C11 | CCAGCCCTTGTGTG | 6 | 0 | No match | -GG | C |
| C12 | CACCATGCCTGGCT | 6 | 0 | cDNA, 3′ end (Hs.155873) | -GC | B |
| C13 | TGGTCTTCGCCTTG | 5 | 0 | No match | -CA | B |
| C14 | TCTGCTGAGTGGGC | 5 | 0 | Aldehyde dehydrogenase 10 (ALDH10) | -CC | B |
| C15 | TCCAGCCTGGGCTA | 5 | 0 | cDNA, 3′ end (Hs.53106) | -GA | C |
| C16 | CGTCGAGGTCAGTC | 5 | 1 | cDNA, 3′ end (Hs.30807) | -CA | C |
| C17 | CCCAAGGCCAGGCC | 5 | 0 | No match | -GC | B |
| C18 | CCAGACTGCCCACC | 5 | 0 | No match | -GC | B |
| C19 | CACCACTCACAGCT | 5 | 0 | Aldo-keto reductase family 7, member A2 | -CG | B |
| C20 | AGGTGCCCTCTGTG | 5 | 0 | Laminin receptor 1 (67 kDa, ribosomal protein SA) | -AG | C |
a
FC=follicular carcinoma; FA=follicular adenoma.
b
A=a positive result in the second screening; B=a negative result in the second screening; C=discordant results in SAGE and RT–PCR; D=no amplification of a single band.