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. 2004 Apr 6;90(9):1863–1870. doi: 10.1038/sj.bjc.6601759

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Copyright © 2004 Cancer Research UK

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Schematic representation of fusion protein expression vector pBJR-2. Ap^R, ampicillin resistance gene; ColE1, origin of DNA replication; c-myc, sequence encoding the c-myc epitope; His₆, hexa-histidine encoding sequence; P/O, lac wild-type promoter/operator; rbs, ribosome-binding site; pelB, signal peptide sequence of bacterial pectate lyase; V_H, variable heavy chain; V_L, variable light chain; rapLR1, Rana pipiens liver ribonuclease 1; H71, framework 3 residue 71; G₄S, spacer connecting the ribonuclease with the scFv; (G₄S)₃, linker connecting the variable domains. Restriction sites for cloning are indicated.