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. 2004 Apr 6;90(9):1833–1841. doi: 10.1038/sj.bjc.6601760

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Copyright © 2004 Cancer Research UK

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Nuclear localisation of PBGD in B16 melanoma cells (A) In immuno-labelling, the nuclear versus cytoplasmic distribution of PBGD was similar for all experiments. In control cells, in which anti-PBGD antibody was withdrawn from the samples, there was no signal. Original magnification X 60, bar 5 μm. (B,C) Over expression of human housekeeping PBGD fused to GFP on the N-terminal (B) or the C-terminal (C). (D) The negative control for nuclear GFP localisation was cells expressing the wild type GFP plasmid and revealing diffused fluorescence. Original magnification X 100, bar 5 μm.