Figure 2.

Effect of inhibitors of PKC on PIF-induced chymotrypsin-like enzyme activity. Myotubes were incubated with PIF alone (×) or with Ro 31-8220 (1 μM) (A); staurosporine (300 nM) (B); calphostin C (300 nM) (C); or with Go 6976 (200 μM) (D) added 2 h prior to PIF, and chymotrypsin-like enzyme activity was determined 24 h after addition of PIF. The experiment was repeated three times (n=9). Differences from control are indicated as ^aP<0.05 and ^bP<0.001, while differences from cells incubated in the presence of PIF alone are shown as ^cP<0.05, ^dP<0.01 and ^eP<0.001.