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. 2004 Apr 6;90(9):1850–1857. doi: 10.1038/sj.bjc.6601767

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Copyright © 2004 Cancer Research UK

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Time course for induction of chymotrypsin-like enzyme activity in wild-type myotubes (×) and in those transfected with constitutively active (pCO₂ □) and mutant (pKS1 ▪) PKC_α. Myotubes were treated with the indicated concentrations of PIF, and enzyme activity was determined at 3 h (A), 6 h (B), 24 h (C) and 48 h (D). The experiment was repeated three times (n=9). Differences from control or pKS1 are indicated as ^aP<0.05, ^bP<0.01 and ^cP<0.005, while differences from wild-type myotubes are indicated as ^dP<0.005.