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. 2004 Nov 9;91(12):2071–2078. doi: 10.1038/sj.bjc.6602222

Table 1. Expression and methylation primer sequences and PCR conditions.

Primer name Primer sequence (5′ to 3′) Annealing temp. for PCR (°C) MgCl2 conc. (mM) Predicted product size (bp)
SLIT3 COBRA F GGT-TAG-TTT-ATT-YGG-TYG-TTT-YGT-G TT-TTA-GT 55 1.5 394
SLIT3 COBRA R TAC-CCA-CCC-RAA-AAC-CAT-AAT-ATA-CAA-AA      
SLIT3 COBRA RN CCA-CTC-CTA-AAA-AAA-ACT-ACC-TCT-A      
         
SLIT1 COBRA F GTT-TAT-TTT-TTT-TTT-YGT-AGT-AGT-TAG-TTG-GGA-GT 57 1.5 370
SLIT1 COBRA R TAA-CAA-TCC-ACC-RTA-ATT-CCR-ATA-CAA-ATA-CAA-AAA      
SLIT1 COBRA FN GGY-GAA-AYG-GTA-GAG-GAG-TYG-AGT-TTT-T      
         
GAPDH expression F TGA-AGT-TCG-GAG-TCA-ACG-GAT-TTG-GT 60 1.5 982
GAPDH expression R CAT-GTG-GGC-CAT-GAG-GTC-CAC-CAC      
         
SLST3 expression F CAA-GTG-TGC-CGA-GGG-CTA-TGG-AG 65 1.5 419
SLIT3 expression R ACG-GG C-TTA-GGA-ACA-CGC-GAG-G      
         
SLIT1 expression F GTG-ACA-ACT-GCA-GTG-AGA-AC 55 1.5 422
SLIT 1 expression R GTA-CAG-CTC-AAC-TGC-AAT-GT