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. 2004 Dec 14;91(12):2042–2047. doi: 10.1038/sj.bjc.6602254

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Copyright © 2004 Cancer Research UK

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Effect of FCS on Id-1 expression in ovarian cancer cell lines and Id-1 expression in stable transfectants. (A) Western blotting analysis of Id-1 protein expression in five ovarian cancer cell lines, Ovca420, Ovca432, Ovca433, Ovca429 and Skov3, in culture medium containing different serum concentrations. Note that the expression of Id-1 is dependent on FCS in Ovca420, Ovca432 and Ovca433 cell lines but independent in Ovca429 and Skov3 cell lines. (B) Three cell lines Ovca420, Ovca432 and Ovca433 were transfected with an Id-1 expression vector (pBabe–Id-1) or the empty control (pBabe) and stable transfectants were generated. Id-1 protein expression was examined by Western blotting after culturing in SFM for 48 h. Expression of actin was examined as an internal loading control. Note that Id-1 protein levels are much higher after ectopic expression of the pBabe–Id-1 vector in Ovca420, Ovca432 and Ovca433 cell lines. Results represent three experiments.