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. 2004 Dec 14;91(12):2042–2047. doi: 10.1038/sj.bjc.6602254

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Copyright © 2004 Cancer Research UK

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Effect of ectopic Id-1 expression on EGFR. (A) EGFR protein expression in the Id-1 transfectants and the vector control cells were cultured in SFM for 48 h and EGFR expression was examined by Western blotting. (B) EGFR promoter activity in Ovca420, Ovca432 and Ovca433 cells transiently transfected with pcDNAId-1 and the vector control pcDNA. pER-1 (luciferase reporter containing the EGFR promoter) and pRL-CMV-Luc (internal control) was cotransfected with pcDNA or pcDNA-Id-1, respectively. Cells were lysed 48 h after transfection and assayed for luciferase activity using the Dual-luciferase reporter assay system (Promega, WI, USA). The luciferase activity of the cells transfected with pER-1 and pcDNA was considered as 100%. Each experiment was repeated three times and the error bars represent the standard deviation from three independent experiments. Note that ectopic expression of Id-1 in Ovca420, Ovca432 and Ovca433 cells induces upregulation of EGFR at both transcriptional and protein levels.