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. 2004 Dec 14;91(12):2042–2047. doi: 10.1038/sj.bjc.6602254

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Copyright © 2004 Cancer Research UK

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Effect of Id-1 inactivation on EGFR. (A) Id-1 and EGFR protein expression in ovarian cancer cells transiently transfected with an antisense Id-1 expression vector (pcDNA-Id-1-AS) and the control vector (pcDNA). Cells were cultured in SFM for 48 h after transfection and Id-1 and EGFR expression was examined by Western blotting. (B) EGFR promoter activity in ovarian cancer cells transiently transfected with Id1-AS and the vector control. pER-1 (luciferase reporter containing the EGFR promoter) and pRL-CMV-Luc (internal control) were cotransfected with pcDNA or pcDNA-Id-1-AS, respectively, to Skov3 and Ovca429 cells. Cells were lysed for luciferase assays 48 h after transfection and the luciferase activity was tested using the Dual-luciferase reporter assay system (Promega, WI, USA). Samples transfected with pER-1 and pcDNA was considered as 100%. The error bars represent standard deviation from three independent experiments. Note that inhibition of Id-1 expression in Skov3 and Ovca429 cells reduces EGFR expression at both transcriptional and protein levels.