Skip to main content
. 2004 Jun 15;91(2):262–269. doi: 10.1038/sj.bjc.6601949

Table 1. Conditions for immunohistochemistry.

Antibody Source Dilution Incubation Ag retrieval
Ki67 Novocastra 1 : 25 1 h RT MW 20 min Na citrate pH 6
p21 DAKO 1 : 25 1 h RT MW 20 min Na citrate pH 6
Caspase 3 R&D systems 1 : 600 O/N 4°C MW 20 min Na citrate pH 6
Mcm2 Transduction laboratories 1:1000 1 h RT PC 2 min Na citrate pH 6
G92 See text      
G95 See text      

RT=room temperature, O/N=overnight, MW=microwave, PC=pressure cooker.