Table 1. Conditions for immunohistochemistry.
Antibody | Source | Dilution | Incubation | Ag retrieval |
---|---|---|---|---|
Ki67 | Novocastra | 1 : 25 | 1 h RT | MW 20 min Na citrate pH 6 |
p21 | DAKO | 1 : 25 | 1 h RT | MW 20 min Na citrate pH 6 |
Caspase 3 | R&D systems | 1 : 600 | O/N 4°C | MW 20 min Na citrate pH 6 |
Mcm2 | Transduction laboratories | 1:1000 | 1 h RT | PC 2 min Na citrate pH 6 |
G92 | See text | |||
G95 | See text |
RT=room temperature, O/N=overnight, MW=microwave, PC=pressure cooker.