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. 2004 Jun 29;91(3):564–571. doi: 10.1038/sj.bjc.6601990

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Copyright © 2004 Cancer Research UK

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(A) Quantitative RT–PCR of mRNA derived from microdissected MEs of normal breast tissue, low- and high-grade DCIS using PAI-1-specific primers. From each group one case was selected and a LightCycler™ analysis was performed in triplicate. The amount of PAI-1 mRNA was calculated by assorting each CP to a standard curve. (B) The RNA of MEs (DCIS and normal breast tissue) was isolated, reverse transcriptase reaction followed by a PCR using uPAR primers (see Materials and methods) was performed. The RT–PCR reveals a 311 bp product in both probes (DCIS and normal breast tissue); without RT reaction no product was received.