Table 1. Summary of MS-PCR parameters.

		Gleason score
(a)	# of genes hypermethylated	1–4	5–7	8–10
P=0.1781	0–1	3	28	14
	2–3	0	15	10
	4	1	34	8
		Tumour stage
		pT1–2	pT3–4
P=0.2024	0–1	24	21
	2–3	10	15
	4	15	28
		Lymph node status
		N0	N+
P=0.4817	0–1	39	6
	2–3	19	6
	4	34	9
		Gleason score
(b)	Hypomethylation	1–4	5–7	8–10
P=0.5937	0–3%	1	40	14
	4–12%	2	24	9
	>12%	1	13	9
		Tumour stage
		pT1–2	pT3–4
P=0.04187	0–3%	29	26
	4–12%	15	20
	>12%	5	18
		Lymph node status
		N0	N+
P=0.08172	0–3%	47	8
	4–12%	30	5
	>12%	15	8

For each gene the primer sequences used, the annealing temperatures, and the number of cycles are indicated. M=methylated-specific primers; U=unmethylated-specific primers; BS=primers used for bisulphite sequencing.