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| Gene | MgCl2 (mM) | Annealing temp. (°C) | Elongation time (s) | Detect temp. (°C)a |
|---|---|---|---|---|
| Type I collagen | 2.25 | 63 | 15 | 88 |
| Type III collagen | 4 | 63 | 10 | 80 |
| TGF-β | 3 | 65 | 8 | 86 |
| CTGF | 2.25 | 66 | 13 | 85 |
| aFGF | 4 | 53 | 5 | 81 |
| bFGF | 5 | 58 | 7 | 72 |
| PDGF A | 3 | 68 | 10 | 88 |
| PDGF C | 3 | 56 | 9 | 79 |
| EGF | 4 | 65 | 13 | 72 |
Temperature for detection of fluorescent PCR products.
