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| PCR | Primer sequence | [Primer] (μM) | [MgCl2] (mM) | Size (bp) |
|---|---|---|---|---|
| ERα | AGACATGAGAGCTGCCAACC | 2 | 1.5 | 299 |
| GCCAGGCACATTCTAGAAGG | ||||
| ERβ1 | CCAAATGAGGGACCACACAGCAG | 1 | 1.5 | 476 |
| AGTATGTACCTCTGGTCACAGCG | ||||
| β-actin | TGACGGGGTCACCCACACTGTGCCCATCTA | 0.48 | 1.5 | 610 |
| CTAGAAGCATTTGCGGTGGACGATGGAGGG | ||||
| HPRT | CTATTGTAATGACCAGTCAACAGGGG | 1 | 3 | 367 |
| AACTCAACTTGAACTCTCATCTT |
