Table 3. Conditions of sequence-specific PCR cycling reactions.

		Condition
Oligonucleotide	Cycle	Denaturation phase	Annealing phase	Extension phase
ERα and β-actin	35	94°C, 15 s	58°C, 15 s	72°C, 30 s
ERβ1	40	94°C, 30 s	64°C, 40 s	72°C, 45 s
HPRT	36	94°C, 30 s	60°C, 60 s	—