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. 2004 Oct 19;91(9):1678–1686. doi: 10.1038/sj.bjc.6602161

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Copyright © 2004 Cancer Research UK

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(A) A SSCP analysis of exon 8 of the p53 gene run on 12% polyacrylamide gel with 1% glycerol at 10 W for 16 h. Note band shift in lane marked B 16 (indicating a p53 alteration). The shift is a result of conformational changes induced by the point mutation identified in the sequencing gel in (B). (B) DNA sequence analysis of exon 8 for sample B 16 indicating base pair substitution at codon 273 changing an arginine residue to histidine. Nucleotide base pairs: A=adenine; G=guanine; C=cytosine; T=thymine.