Figure 5.
Clodronate downregulated oestrogen receptor expression as assessed by Western blot. MCF-7 cells were incubated for 24 h with 10−4 M clodronate (Clod), 10−9 M E2, Clod+E2 or vehicle (control) in SFM. Equal quantities of proteins (20 μg) were subjected to 10% SDS–PAGE and electrotransferred onto nitrocellulose membranes. Immunodetection was performed with anti-human ER antibody raised against its F domain. Blots show representative experiments performed twice. Quantitative data were obtained from densitometric analyses and are presented as percentages of control values (mean). ND=not detectable.