Figure 7.

Khat-induced cell death in HL-60 cells was dependent on de novo protein synthesis. Cells were exposed to 200 μg ml⁻¹ khat in the absence (•) or presence of 31.6 ng ml⁻¹ CHX (▾) or 100 ng ml⁻¹ CHX (▪). Some cultures were left unsupplemented (○) as controls or treated with 31.6 ng ml⁻¹ CHX (▿) or 100 ng ml⁻¹ CHX (□). CHX was added 15 min prior to treatment with khat. At the times indicated, cell aliquots (50 μl) were removed and dead cells determined as the fraction of cells (%) having condensed nuclear chromatin. The data represent means±s.e.m. of two separate experiments, each in triplicate.