Table 1.
Effect of agents on Ca2+ wave propagation in the rat eyecup
| Condition | Astrocyte wave radius (μm) | Müller cell wave radius (μm) | Astrocyte → Müller cell delay (sec) |
|---|---|---|---|
| Astrocyte stimulation | |||
| Control | 84.7 ± 3.2 (24) | 82.2 ± 3.4 (24) | 0.85 ± 0.09 (18) |
| Octanol (0.5 mm) | 100.6 ± 3 (20)* | 100.6 ± 3 (20)* | 0.99 ± 0.06 (12) |
| Suramin (100 μm) | 54.5 ± 6.2 (21)* | 12.9 ± 5.2 (21)* | 2.38 ± 0.13 (4)* |
| PPADS (20 and 50 μm) | 69.0 ± 3.7 (33)* | 49.8 ± 6.0 (33)* | 2.14 ± 0.14 (22)* |
| Apyrase (80 U/ml) | 63.3 ± 9.6 (9)* | 20.7 ± 6.8 (9)* | |
| Müller cell stimulation | |||
| Control (eyecup)1-a | 64.4 ± 2.2 (18) | ||
| Control (slice) | 66.0 ± 3.0 (28) | ||
| Suramin (slice, 100 μm) | 32.6 ± 3.5 (22)* |
Calcium waves were evoked by mechanical stimulation. Wave radii were measured 9.5 sec after stimulation. The last column gives the time between the onset of a Ca2+ increase in an astrocyte process and the onset of an increase in an adjacent Müller cell endfoot. Mean ± SEM (n) are given.
*
p < 0.01; control versus test.
F1-a
Wave radius measured at the retinal surface.