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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Exp Cell Res. 2008 Mar 5;314(8):1789–1803. doi: 10.1016/j.yexcr.2008.02.014

Fig. 6.

Fig. 6

PGAM5 contributes to repression of Nrf2-dependent gene expression. (A) Anti-Nrf2 immunoprecipitates from 9.5 mg of HEK-293T cell lysates (lane 2) were subjected to immunoblot analysis with anti-PGAM5 (top panel) and anti-Nrf2 antibodies (bottom panel).(B) Hela cells were transfected an ARE-dependent firefly luciferase reporter gene construct (0.1 μg) and with siRNA nucleotides (300 nM) against Nrf2, Keap1, or PGAM5 as indicated. A plasmid encoding Renilla luciferase (0.01 μg) was included as a control for transfection efficiency. The data shown are the mean and standard deviation from the mean of three independent experiments.