Figure 4.

The GST-AtCDC5 fused protein possessed a sequence specific DNA binding activity. (A) Summary of the nucleotide sequence of DNA fragments recognized by the GST-AtCDC5 fused protein in random binding site selection experiments. The base constitution around the consensus sequence was indicated in percent. (B) Gel mobility shift assay. Five nanograms per lane of the GST-AtCDC5 protein was used. C, A DNA fragment with the consensus sequence (68-mer left arm-ACCAACGGGCGCTGAGCTGATGTCG-68-mer right arm); N, a DNA fragment without the consensus sequence (68-mer left arm-ACACCAAGTCTTACGCGCTGTCTCGC-68-mer right arm).