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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Clin Cancer Res. 2008 Jun 1;14(11):3617–3626. doi: 10.1158/1078-0432.CCR-07-2060

Figure 2. The combined treatment of Ad-MMP-9 and radiation decreases binding activity of NF-κB and AP1.

Figure 2

(A) Western blot results for ERK1/2 and pERK. GAPDH was used as a control to confirm equal loading of cell lysates. (B) Western blot analysis of sub-cellular fractions showing the translocation of the p50 and p65 subunits of NF-κB and c-fos and Jun D subunits of AP1. (C) The binding activities of NF-κB and AP1 to DNA were determined using the electromobility shift assay. Oligonucleotide consensus probes for NF-κB and AP1 were end labeled with 32P and used in the shift assay. (IR-Irradiation, SS-Supershift)