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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Clin Cancer Res. 2008 Jun 1;14(11):3617–3626. doi: 10.1158/1078-0432.CCR-07-2060

Figure 3. Downregulation of MMP-9 and radiation induces apoptosis in breast cancer cells.

Figure 3

(A) Western blot results for Fas and Fas-L. Representation of densitometric analysis of the expression of of Fas and Fas-L (mean ± S.E.; n=4). (B) Western blot results for caspases 10, 3 and 7 show active caspase and PARP cleavage. (C) & (D) TUNEL assay was performed with MDA MB 231 cells with the earlier said treatments as per the manufacturer’s protocol and the densitometric analysis of percentage of TUNEL positive cells are represented (mean ± S.E.; n=3). *, Significant difference between irradiated alone cells and combination treatment of Ad-MMP-9 infection with irradiation (P ≤ 0.05). (E) Clonogenic survival was assessed between 15 d after radiation exposure. Each experiment was done at least four times and triplicates were done for each experiment. Clonogenic survival was assessed between 15 d after radiation exposure. Each experiment was done at least four times and triplicates were done for each experiment. Statistical analysis was done using PRISM and ANOVA. *, Significant difference between irradiated alone cells and combination treatment of Ad-MMP-9 infection with irradiation (P ≤ 0.01). (IR-Irradiation)