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. Author manuscript; available in PMC: 2008 Jun 4.
Published in final edited form as: J Immunother. 2003;26(1):85–93. doi: 10.1097/00002371-200301000-00009

FIG. 4.

FIG. 4

Effects of IL-2 on CD4+CD25+ T cell-mediated suppression. (A) CD4+CD25+, CD4+CD25−, or a 1:1 ratio of CD4+CD25+:CD4+CD25−T cells were stimulated with 5 μg/mL anti-CD3 and 1 × 104 T cell-depleted accessory cells for 7 days. (B) Identically treated cells were also incubated with 300 IU/mL of human recombinant IL-2. [3H]thymidine was added to culture for the final 18 hours of culture. IL-2 reversed the hyporesponsive state of CD4+CD25+ T cells and largely abrogated suppression mediated by these cells. Conditions were done in sextuplicates, and error bars represent SEM.