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. Author manuscript; available in PMC: 2008 Jun 4.
Published in final edited form as: J Immunol. 2002 Nov 15;169(10):6036–6047. doi: 10.4049/jimmunol.169.10.6036

FIGURE 2.

FIGURE 2

Multiple recognition patterns of autologous cells by CD4-positive T cell clones. The CD4-positive T cell lines (2 × 104 cells/well) were incubated with 105 1290 mel cells that were untreated or that had been treated for 72 h with 500 U/ml of IFN-γ, which are indicated by (+), and extensively washed before addition to the T cell clones. In addition, 2 × 105 EBV B cells were pulsed overnight with 2 × 105 1290 mel cells that were lysed by three successive freeze thaws before addition of T cells. Targets were incubated with 2 × 104 T cells overnight, and the release of IFN-γ was measured in an ELISA.