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. 2004 Oct 26;91(10):1813–1820. doi: 10.1038/sj.bjc.6602189

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Copyright © 2004 Cancer Research UK

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Validation of the ΔΔ Ct method for the quantification of hMAM results: (A) amplification plots obtained by the ABI Prism 7700 for hMAM mRNA expression; (B) Standard curve for hMAM and β-Actin constructed of six cDNA dilutions obtained from an MDA-MB361 breast cancer cell line; (C) Plot of the log of input of total RNA vs the ΔCt value (Ct hMAM - Ct β-Actin).