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. 2004 Oct 19;91(10):1842–1850. doi: 10.1038/sj.bjc.6602193

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Copyright © 2004 Cancer Research UK

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Colonies of hygromycin-resistant prostate epithelial cells expressing clusterin are smaller than those expressing only hyg^r. Cell colonies selected in cell culture dishes, fixed with paraformaldehyde 4% and stained with Giemsa. Note the smaller size of the clu colonies (clu) compared to the hyg colonies (hyg), after 14 days of selection with hygromycin B. The figure shows a representative result obtained by performing the experiment four times using different standard endo-free plasmid preparations.