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. 2007 Dec 17;104(52):20764–20769. doi: 10.1073/pnas.0705659105

Table 1.

Kinetic analysis for human AKRs and mutant enzymes using the BSA/HPLC method

	d,l-Glyceraldehyde			all-trans-Retinaldehyde			9-cis-Retinaldehyde
	K_m, mM	k_cat, min⁻¹	k_cat/K_m, mM⁻¹·min⁻¹	K_m, μM	k_cat, min⁻¹	k_cat/K_m, mM⁻¹·min⁻¹	K_m, μM	k_cat, min⁻¹	k_cat/K_m, mM⁻¹·min⁻¹
AKR1B1	0.05 ± 0.01	31 ± 1	660 ± 80	1.1 ± 0.1^*	0.35 ± 0.01^*	320 ± 30^*	0.4 ± 0.1	0.7 ± 0.1	1,500 ± 170
AKR1B10	6 ± 1	35 ± 1	6 ± 1	0.6 ± 0.1^*	27 ± 1^*	45,000 ± 7,600^*	0.7 ± 0.1	0.9 ± 0.1	1,300 ± 160
AKR1B10 K125L	5.2 ± 0.8	35.6 ± 0.1	7 ± 1	0.15 ± 0.02	2 ± 0.06	13,100 ± 2,000	0.7 ± 0.1	0.9 ± 0.1	1,300 ± 190
AKR1B10 V301L	6.0 ± 0.7	41 ± 1	7 ± 1	0.6 ± 0.1	7.7 ± 0.3	12,700 ± 1,400	0.30 ± 0.03	0.60 ± 0.01	1,800 ± 140
AKR1B10 K125L/V301L	7.3 ± 0.7	35.4 ± 0.9	4.9 ± 0.5	1 ± 0.3	3.5 ± 0.01	3,500 ± 320	ND	ND	ND

ND, not determined.

*Data taken from ref. 7.