Fig. 3.

c₁₁-ring formation assisted by uncI without involvement of other subunits of F₁F_o. SDS/PAGE of membrane fractions prepared from cells expressing pTR-I_Hisc (lane 2), pTR-c (lane 3), and pTR-c + pST-I_His (lane 4). Lane 1, purified hF₁F_o⁽⁺ⁱ⁾ as a reference. Membrane proteins (7 μg) were applied to each lane of the gels. Bands of c₁₁-ring and c-monomer are indicated with arrowheads. It should be noted that expression of UncI from pST-I_His is weaker than that from pTR-I_Hisc because of a weaker transcription promoter.