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. 2007 Dec 19;104(52):20782–20787. doi: 10.1073/pnas.0708909105

Fig. 1.

Fig. 1.

TOXCAT and SDS/PAGE analysis of syndecan TMD self-association. (A) Sequences of the ToxR(TMD)MBP fusions of human syndecan-1, syndecan-2, syndecan-3, and syndecan-4. (B) TMDs plus flanking regions of the human syndecans fused to the C-terminal end of SNase (bold) to yield the SNSyn1, SNSyn2, SNSyn3, and SNSyn4 fusion proteins. (C) (Upper) CAT activity from cells expressing the TXSyn1, TXSyn2, TXSyn3, and TXSyn4 constructs. Error bars show standard deviation of three independent cultures. GpA (TXGpA) and its disruptive mutant (TXGpA83I) serve as positive and negative controls. (Lower) ToxR(TMD)MBP expression levels measured by Western blot are similar for all cultures. (D) MalE complementation assay of ToxR fusions. Untransformed malE cells (untrans) or those carrying an empty vector (pccKAN) do not grow with maltose as the sole carbon source, but the control TXGpA or syndecan fusions permit robust growth, indicating that the MBP domains are directed to the periplasm. (E) SDS/PAGE of the purified SNSyn1, SNSyn2, SNSyn3, and SNSyn4 fusion proteins. Markers and migration positions consistent with the molecular masses of protein monomers and dimers are indicated.