Table 1. Production of GHRH in culture medium from human breast cancer (MCF-7, MDA-MB-468, MDA-MB-435S, T47D) prostate cancer (LNCaP) and non-small cell lung carcinoma (NCI H838) cell lines.
|
GHRH (ng ml−1 medium) produced by CANCER CELL LINES
|
||||||
|---|---|---|---|---|---|---|
| Time (hour) | MCF-7 | T47D | MDA-MB-468 | MDA-MB-435s | LNCaP | NCI H838 |
| 0 | ND | ND | ND | ND | ND | ND |
| 24 | ND | 1.518 | 0.637 | 0.675 | 0.202 | 0.303 |
| 48 | ND | 2.083 | 0.816 | 1.036 | 0.218 | 0.442 |
| 72 | ND | 0.266 | 0.649 | 1.442 | 0.208 | 0.689 |
ND=not detectable.
Aliquots of medium were subjected to RIA for the detection of GHRH at the indicated periods of time.