Table 3.
Distribution of the most abundant secreted genes (≥ 5 EST) among the four libraries
| IRUF | IR24H | IR4D | IR7D | ||||||||||||
| p | p | p | p | ||||||||||||
| Name of group | Contigs | EST | obs | exp | IRUF/IR24H | obs | exp | IR24H/IR4D | obs | exp | IR4D/IR7D | obs | exp | ALL | |
| CLSP | 71 | 209 | 2 | 53.67 | 0.000 | 104 | 54.67 | 0.000 | 62 | 52.67 | 0.102 | 41 | 48.00 | 0.000 | |
| BTSP | 46 | 95 | 6 | 24.39 | 0.000 | 48 | 24.85 | 0.000 | 17 | 23.94 | 0.135 | 24 | 21.82 | 0.000 | |
| Kunitz domain | 30 | 61 | 0 | 15.66 | 0.000 | 33 | 15.96 | 0.000 | 19 | 15.37 | 0.104 | 9 | 14.01 | 0.000 | |
| 18.7 kDa | 11 | 20 | 2 | 5.14 | 0.001 | 12 | 5.23 | 0.003 | 6 | 5.04 | 0.029 | 0 | 4.59 | 0.001 | |
| Metalloproteases | 13 | 18 | 3 | 4.62 | 0.276 | 3 | 4.71 | 0.409 | 4 | 4.54 | 0.055 | 8 | 4.13 | 0.185 | |
| WC proteins | 15 | 18 | 0 | 4.62 | 0.031 | 5 | 4.71 | 0.244 | 7 | 4.54 | 0.140 | 6 | 4.13 | 0.075 | |
| Ixodegrins | 8 | 15 | 0 | 3.85 | 0.000 | 10 | 3.92 | 0.002 | 4 | 3.78 | 0.186 | 1 | 3.44 | 0.002 | |
| 19 kDa | 4 | 14 | 0 | 3.59 | 0.000 | 13 | 3.66 | 0.000 | 1 | 3.53 | 0.025 | 0 | 3.22 | 0.000 | |
| HBP | 10 | 10 | 0 | 2.57 | 0.059 | 1 | 2.62 | 0.172 | 4 | 2.52 | 0.044 | 5 | 2.30 | 0.054 | |
| ISAC-like | 7 | 7 | 0 | 1.80 | 0.178 | 2 | 1.83 | 0.348 | 3 | 1.76 | 0.327 | 2 | 1.61 | 0.431 | |
| Prokineticin domain | 7 | 7 | 0 | 1.80 | 0.111 | 3 | 1.83 | 0.378 | 2 | 1.76 | 0.721 | 2 | 1.61 | 0.455 | |
| Salp15-like | 7 | 7 | 0 | 1.80 | 0.037 | 4 | 1.83 | 0.089 | 1 | 1.76 | 0.514 | 2 | 1.61 | 0.194 | |
| 6.78 kDa | 4 | 6 | 0 | 1.54 | 0.198 | 2 | 1.57 | 0.208 | 3 | 1.51 | 0.210 | 1 | 1.38 | 0.360 | |
| 4.9 kDa | 1 | 5 | 0 | 1.28 | 0.107 | 0 | 1.31 | 0.109 | 0 | 1.26 | 0.000 | 5 | 1.15 | 0.001 | |
P values shows significance of expression difference between two libraries; P – ALL shows which contig has random and non-random distribution. Significant values are (P ≤ 0.05) are marked in bold. Obs – observed, Exp – expected.