Table 1. Primary antibodies and conditions.

Antibody against	Source	Cat. no.	Dilution	Pretreatment
PSA	DAKO, CA, USA	M0750	1 : 20	Microwave 2 × 5 mina
c-erbB-1	DAKO, CA, USA	M3563	1 : 100b	Pronase 10 min
c-erbB-2	Novocastra Laboratories Ltd, Newcastle, UK	NCL-CB11	1 : 200	Microwave 2 × 5 min
c-erbB-3	SantaCruz Biotechnology Inc., CA, USA	SC-415	1 : 50	None
c-erbB-4	SantaCruz Biotechnology Inc., CA, USA	SC-283	1 : 50	Microwave 2 × 5 min

^aIn 10 mmol/l citrate buffer (pH 6.0).

^bApplied overnight.