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. 2004 Feb 17;90(4):926–931. doi: 10.1038/sj.bjc.6601583

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Copyright © 2004 Cancer Research UK

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(A) Western analysis for Hsp70 in supernatants of pcDNA3.1+Hsp70 and Renilla luciferase or mock-transfected TRAMP-C2 cells. Spent media were collected at 24, 48, and 72 h and concentrated as in Materials and methods. (B) Western analysis for Hsp70 in cell extracts of Hsp70, Renilla luciferase, and mock-transfected TRAMP-C2 cells. Ponseau S was used for normalisation. (C) Percent of Hsp70 in supernatants (Sup) and cell extracts (CE) of Hsp70 and mock-transfected TRAMP-C2 cells as determined by densitometry. (D). Comparison of luciferase activity in cell extracts (C) vs supernatants (S) in C2 cells transfected with pcDNA3.1+Hsp70 and Renilla luciferase at various time points. Spent media and whole-cell protein extracts were prepared as above. (E) Percentage of renilla protein was determined by luminescence.