Figure 1.

Degradation of ¹²⁵I-labeled AgIgG in LEC, KC and J774 cells. ¹²⁵I-AgIgG (10 micrograms/ml) was added to the cells (10⁶ cells per ml) incubated at 37 degrees C. Aliquots of medium, removed at the indicated time points, were treated with ice-cold trichloroacetic acid and centrifuged at 1000 – g for 10 min. Acid-soluble radioactivity in the supernatant is presented as % of total acid precipitable radioactivity added to the cells at the start of the incubation. At the end of the incubation (240 min) about 1.5% of the total radioactivity was present in the cells. The results are given as means – S.D. of 3 experiments.