Figure 2.

Anti-Fc gamma R IgG inhibits binding of ¹²⁵I-AgIgG. LEC, KC and J774 cells were incubated with mouse anti rat CD32 (KC and LEC) or rat anti-mouse CD16/32 (J774 cells) antibodies (10 micrograms/ml) on ice. ¹²⁵I-AgIgG (10 micrograms/ml) was then added, and cell-associated radioactivity was measured after incubation on ice for 2 hours. The results represent % of control (binding in absence of antibodies) for one typical experiment.