Skip to main content
. 1999 Nov 23;96(24):13650–13655. doi: 10.1073/pnas.96.24.13650

Figure 1.

Figure 1

(A) Structure of the BMV RNA genome and plasmid DNA cassettes used to express wild-type BMV RNA2 and CP. GAL1, Rz, and A+ denote the GAL1 promoter, a self-cleaving hepatitis δ virus ribozyme (18), and the yeast ADH1 polyadenylation signal, respectively (14). (B) BMV RNA and protein accumulation in yeast. Equal amounts of total RNA from yeast expressing the indicated BMV components were analyzed by Northern blotting with RNA2 (Top) and CP (Second blot) probes. RNA from plant-derived BMV virions (BMVP) was used as a control. Equal amounts of total protein from yeast expressing the indicated components were analyzed by Western blotting with anti-2a (Third blot) and anti-CP (Bottom) antisera. Controls (lane 5) were baculovirus-expressed 2a protein (19) (Third blot, lane 5) and BMVP CP (Bottom, lane 5).