Figure 4.
CryoEM and three-dimensional image reconstruction of CCMV, BMVP, and yeast-derived VLPs. The 88S and 65S VLPs were purified by sucrose gradient sedimentation from yeast expressing RNA2 + CP and CP only, respectively. (A) Cryoelectron micrographs showing representative fields of the indicated particles. Unstained particles appear dark against the brighter (less dense) supporting layer of vitreous ice. (B) Three-dimensional image reconstructions of CCMV (7), BMVP, and the 88S and 65S VLPs from yeast.