Table 1.
Particle | s20,w, S | Mr (×106)* | Mr (×106)† | % RNA‡ |
---|---|---|---|---|
BMV (ref. 16) | 88 | 4.6 | NA | 21.4 |
BMVP (this work) | 88 | 4.3 ± 0.2 | 4.5 | 20.0 ± 1.2 |
65S VLP | 65 | 2.7 ± 0.2 | 2.7 | 9.5 ± 1.4 |
Determined by analytical ultracentrifugation.
† Determined by scanning transmission electron microscopy. Data generously provided by M. Simon at Brookhaven National Laboratory (29).
‡ Determined from sucrose gradient-purified particles. Protein content was measured using the Hartree–Lowry or bicinchoninic acid (Pierce) assay. Extracted RNA content was measured by optical absorbance at 260 nm.