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. 1999 Nov 23;96(24):13656–13661. doi: 10.1073/pnas.96.24.13656

Figure 1.

Figure 1

Immunoblot analysis of SREBP-1 and -2 in membranes and nuclear extracts from livers of control rats and rats treated with STZ without or with INS. Livers from the three groups of rats in Table 1 (four male rats per group) were pooled, and aliquots of the membrane pellet (30 μg of protein) and nuclear extract (30 μg) were subjected to SDS/PAGE (8% gel). Immunoblot analysis was performed with 5 μg/ml rabbit anti-rat SREBP-1 IgG (lanes 1, 2, and 3) or anti-rat SREBP-2 IgG (lanes 4, 5, and 6) as the primary antibody and 0.25 μg/ml horseradish peroxidase-coupled donkey anti-rabbit IgG as the secondary antibody. Filters were exposed to Reflection NEF496 film for 15 s (lanes 1, 2, and 3) or 30 s (lanes 4, 5, and 6) at room temperature. P and N denote the precursor and cleaved nuclear forms of SREBP, respectively.