Figure 2.

Amounts of various mRNAs in livers of control rats and rats treated with STZ without or with INS as measured by blot hybridization. The rats used in this experiment are described in Table 1. For each experimental group, total RNA isolated from the livers of four male rats were pooled, and 10 μg aliquots were subjected to electrophoresis and blot hybridization with the indicated ³²P-labeled cDNA probe. The amount of radioactivity in each band was quantified as described in Materials and Methods. The -fold change in each mRNA of the STZ-treated diabetic group (S) and STZ-treated diabetic group supplemented with INS (S+I) relative to that of the control group (C) was calculated after correction for loading differences by measurement of cyclophilin mRNA. These values are shown below each blot.