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. 1999 Nov 23;96(24):13662–13667. doi: 10.1073/pnas.96.24.13662

Figure 4.

Figure 4

The BRE is highly conserved and defines transcription polarity on other archaeal promoters. (a) Alignment of characterized Sulfolobus promoter sequences. R = purine; Y = pyrimidine; lack of conservation is indicated by a horizontal bar. (b) In vitro transcription assays using plasmid constructs with oligonucleotides corresponding to the wild-type S. shibatae 16S rRNA gene TATA box and flanks (16S WT) or with the 6 base pairs flanking the TATA box swapped (16S 6FL). The RNA specifically initiating at the T6 start site in a factor-dependent manner is shown by a filled arrowhead. The open arrowheads indicate factor-independent initiation by the RNAP; see Fig. 1 for details. (c) In vitro transcription assays using plasmid constructs with oligonucleotides corresponding to the wild-type S. shibatae T6 gene TATA box and flanks (T6 WT) or with the 6 base pairs flanking the TATA box swapped (T6 6FL). The RNA specifically initiating at the T6 start site in a factor-dependent manner is shown by a filled arrowhead.